scFlowKit

Apr 2, 2025 ยท 1 min read

scFlowKit is a modular and extensible toolkit written in R for streamlined single-cell RNA-seq analysis.

Designed to support both standard pipelines and customizable workflows, it allows bioinformatics researchers to rapidly process, integrate, and visualize scRNA-seq data.

๐Ÿ”ง Key Features

  • Compatible with both Seurat and SingleCellExperiment objects
  • Includes powerful general-purpose function read_sce() to read .h5, .mtx, .loom, and .h5ad formats
  • Integrated modules for:
    • Quality control
    • Batch correction
    • Dimensionality reduction
    • Clustering & annotation
    • Visualization
  • Structured annotation and CLI prompts for usability

๐Ÿ“ฆ Use Cases

  • Rapid startup for scRNA-seq project prototyping
  • Teaching material for reproducible single-cell workflows
  • Integration with other downstream analysis frameworks

scFlowKit is actively maintained and aims to bridge the gap between flexible scripting and reproducible workflows in single-cell transcriptomics.